The unilateral ureteral obstruction (UUO) model is a widely used model of renal fibrosis. The UUO model shows pathological features of chronic kidney disease such as interstitial fibrosis, tubular atrophy, and inflammatory cell infiltration.
The UUO model has a test duration of 2 weeks, which makes this model suitable for in vivo screening tests of anti-fibrotic drugs for kidney fibrosis.
Our UUO model induction process is shown below. One side of the ureter is ligated to induce the disease. The diseased kidney is divided into three sections for analysis such as histopathology, biochemistry and gene expresstion, so various analyses can be performed using the samples from a single mouse.
Histological images of a UUO model kidney are shown below. On the left is a Sirius Red stained section, which shows that the induction of fibrosis in the cortex of the kidney.
On the right side, PAS stained sections show the kidney damage in the UUO model, including tubular swelling, cast formation and loss of brush border, which are not observed in normal mice. Through our pharmacology study service, we can evaluate therapeutic efficacy using these as primary endpoints.