It has been reported that many liver cancers develop from chronic liver diseases accompanied by fibrosis. The DEN-CCl₄ model is a model that causes liver cancer backed by cirrhosis. Compared to the STAM™ model which has a diabetic background, the DEN-CCl₄ does not induce diabetes and thus can be used for several study purposes. DEN-CCl₄ model is created by a single dose of low-dose genotoxic substance diethylnitrosamine (DEN), followed by repeated doses of carbon tetrachloride (CCl₄), developing 100% liver tumors at 30 weeks of age. This model also allows the assessment of anti-inflammatory, anti-fibrotic and anti-tumor agents.